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. 2017 Mar 20;114(14):E2816–E2825. doi: 10.1073/pnas.1617777114

Fig. 3.

Fig. 3.

HITS-CLIP data. (A–C) Reproducibility of biological replicates. Height of CLIP peaks were log-transformed and then plotted on each axis. Data were colored based on the precipitated protein (Sc_PUF3, blue; Nc_PUF3, red; Nc_PUF4/5, green). Spearman’s correlation coefficients (ρ), associated P values (P), and Pearson’s correlation coefficient (r) are indicated in A (ρ = 0.69, 0.84, 0.64; P = 0, 0, 0; r = 0.77, 0.96, 0.81; n = 500), B (ρ = 0.64, 0.85, 0.76; P = 0, 0, 0; r = 0.99, 0.99, 0.99; n = 835), and C (ρ = 0.85, 0.92, 0.79; P = 0, 0, 0; r = 0.71, 0.97, 0.67; n = 746). (D–G) Examples of binding peaks (read depth) for COX17, a canonical target of Sc_PUF3. YLL009C (COX17), NCU0058 (al-2), and NCU02530 (cox17) are depicted, with ORFs annotated in blue and the likely binding elements displayed beneath. (H) Overlapping CLIP targets for each protein. Only genes with orthologs in S. cerevisiae and N. crassa are included. RPM, reads per million mapped reads.