Fig. 2.

S876 in TRPA1 S5 domain is critical for sensing isoflurane. (A) Alignment of S5 region used in chimeras from mouse, human, and Drosophila TRPA1. Amino acids in dTRPA1 divergent from the mammalian sequence are marked in yellow. (B) Representative I–V traces (−100 to +100 mV) evoked by isoflurane (0.9 mM, blue traces) and AITC (100 µM, black traces) for WT and mutant (S876V, T877L, and S876V/T877L) rTRPA1. (C and D) Mean currents evoked by isoflurane and propofol (1 mM) measured at +100 mV and −100 mV in HEK293 cells expressing WT and mutant rTRPA1. Data are mean ± SEM from three to six cells.