Fig. 5.

Molecular modeling and functional analysis identify TRPA1 residues involved in binding isoflurane and propofol. (A–C) Binding modes of isoflurane (A), propofol (B), and A-967078 (C) in human TRPA1 revealed by molecular docking. Note that Met-912 and Met-953 interact with isoflurane and propofol but not A-967078. (D) Mean currents (measured at +100 mV) evoked by isoflurane (0.9 mM), propofol (1 mM), or menthol (1 mM) in HEK293 cells expressing WT (n = 3) or mutant (n = 6–11) rTRPA1s. (E) Inhibition of AITC responses by A-967079 in WT (n = 5) and mutant (n = 4–6) rTRPA1s.