Fig. S2.
Representative two-electrode voltage-clamp recordings showing the effects of PcTx1 and Hi1a on hASIC1a channels expressed in Xenopus oocytes. Whole-cell currents were elicited by rapidly switching the pH from 7.45 to 6.00 every 60 s (and every 5 min after washout). (A and B) Oocytes were exposed to 10 nM PcTx1 (A) or 10 nM rHi1a (B) at pH 7.45 for 120 s twice. (C) Recovery of hASIC1a currents during peptide washout compared with control oocytes (no peptide applied). The time constant for recovery from inhibition (τoff) was 0.99 min for PcTx1 and 31.8 min for Hi1a. Data are mean ± SEM. PcTx1, n = 3; Hi1a, n = 4.