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. 2017 Mar 20;114(14):E2891–E2900. doi: 10.1073/pnas.1616942114

Fig. S2.

Fig. S2.

Activin-A inhibits allergen-driven effector cytokine release by human CD4+ T cells. Human naive CD4+ T cells were labeled with CFSE and cultured with mitomycin-treated, allergen-loaded, CD3-depleted APCs in the presence of control (PBS) or activin-A for 7 or 9 d (for IL-10). Representative FACS plots show T-cell proliferation in the absence of allergen (A) and cytokine-expressing CD4+ T cells (B) and are representative of n = 8 independent experiments (n = 8 donors). Max, maximum. (C) Real-time PCR analysis of cytokine mRNA expression in CD4+ T cells stimulated as above for 3 d. Results are presented relative to GAPDH and are representative of n = 3 separate experiments (n = 3 donors). (D) Human naive CD4+ T cells were stimulated as in A in the presence or absence of an anti–TGF-β antibody. FACS plots show IL-10–expressing cells and gated CD4+ T cells and represent n = 3 separate experiments (n = 3 donors).