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Differential effects of NRG and EGF on the phosphorylation of coexpressed HER3 and EGFR. NR6 cells stably coexpressing mEos–HER3 and EGFR–GFP were serum starved for 6 h, stimulated with 10 nM EGF or 10 nM NRG for 2–60 min, and subsequently lysed. Western blot analysis of the lysates with the indicated antibodies reveals phosphorylation states of EGFR and HER3 as well as AKT and ERK. Antibodies used are listed in Table S1.
