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. 2017 Mar 20;114(14):E2836–E2845. doi: 10.1073/pnas.1617994114

Fig. 3.

Fig. 3.

Clustering of EGFR and HER3 upon EGF is dependent on EGFR kinase activity. Pairwise distance histograms calculated as in Fig. 2 for the following conditions: (A) Cells expressing the mutant mEos–EGFR–V924R were stimulated with 10 nM EGF for the indicated time and were imaged and analyzed by STORM. (B) Cells expressing mEos–EGFR were treated with gefitinib (10 μM) during the entire period of serum starvation (6 h) and then were stimulated with 10 nM EGF. (C) Cells expressing both mEos–HER3 and EGFR–V924R–GFP were stimulated with 10 nM EGF or 10 nM NRG for 10 min and then were imaged and analyzed by STORM. (D–F) Integrated histograms were calculated from the pair-correlation data as described in Fig. 2 for the mEos–EGFR–V924R mutant and mEos–EGFR with gefitinib. Comparisons with compiled wild-type mEos–EGFR are shown in D and E, and the comparison with wild-type EGFR–GFP coexpressed with mEos–HER3 is shown in F. **P < 0.01; ***P < 0.001.