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. 2017 Apr 12;7:46320. doi: 10.1038/srep46320

Figure 2. Effect of GEBR-32a on cAMP levels.

Figure 2

(a) Quantification of cAMP in HTLA cells. Samples were pre-treated for 10 min with GEBR-32a (32a; 100 μM) or an equal volume of DMSO. Then, 1 μM forskolin (FSK) was added, where indicated, for 20 min. The histogram shows the mean ± s.e.m. for three independent experiments; ***P < 0.001 and *P < 0.05 vs vehicle treated cells; ###P < 0.001 vs FSK alone (ANOVA and post hoc Newman-Keuls test. (b) Quantification of cAMP in rat hippocampal slices. Slices (n = 7 per condition) were pre-treated with increasing amount of GEBR-32a (32a) for 15 min and then stimulated with 0.1 μM forskolin for 15 min. The histogram shows the mean ± s.e.m. for six independent experiments; *P < 0.05 and **P < 0.01 vs samples receiving forskolin alone (ANOVA and post hoc Dunnett’s test).