Table 1.
Epithelial to Mesenchymal Transition Related Gene Expression Changes in Mesothelial Cells in Response to Asbestos Exposure
| Gene | LP9 (24 hours) | LP9 (48 hours) | HPM3 (48 hours) | HPM3 (1 week) | TGF-β |
|---|---|---|---|---|---|
| BMP2 | 3.765 | 3.6387 | 3.7264 | 4.3582 | −1.3692 |
| CDH1 | −6.6193 | −5.5775 | −12.3847 | 1.5624 | −2.3526 |
| COL1A2 | −4.3086 | −4.8255 | −6.3425 | −2.7621 | 6.0221 |
| COL3A1 | −2.2648 | −3.5446 | −7.5456 | −1.415 | 1.1224 |
| F11R | −2.3134 | −3.9013 | −1.9703 | −2.1186 | −1.1799 |
| GNG11 | 3.842 | 4.4137 | 2.6357 | 3.1512 | 1.9597 |
| KRT19 | −7.8025 | −7.2188 | −11.0012 | −1.7105 | −13.8892 |
| KRT7 | −3.121 | −5.5928 | −2.9612 | −1.7818 | −3.3246 |
| MST1R | −2.6243 | −4.0842 | −4.2371 | −2.7908 | −8.288 |
| NODAL | 2.916 | −1.4034 | 3.2188 | 6.2224 | 1.6575 |
| PDGFRB | −6.3478 | −5.982 | −2.6859 | −1.8618 | 1.4718 |
| SNAI1 | −4.2379 | −12.1875 | −1.3492 | 2.147 | 3.1556 |
| SPARC | −2.888 | −4.2795 | −6.402 | −3.1342 | 1.8388 |
| STEAP1 | 1.6883 | 2.4375 | 2.3314 | 3.5186 | −1.3104 |
| TFPI2 | 52.4955 | 51.2382 | 13.0143 | 10.8795 | 4.1882 |
| TGFB2 | −2.1052 | −2.2664 | 3.1156 | 1.5631 | 1.6297 |
LP9 and HPM3 cells were exposed to asbestos (5 μg/cm2) for the indicated times, and whole RNA extracted from these cells was analyzed for gene expression levels of the epithelial to mesenchymal transition pathway proteins in an epithelial to mesenchymal transition PCR array. Transforming growth factor (TGF)-β (10 ng/mL) was used as a positive control.