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. 2017 Feb;187(2):418–430. doi: 10.1016/j.ajpath.2016.10.022

Figure 1.

Figure 1

Lipid peroxidation product adducts; reactive oxygen species generation; and markers of stellate cell activation, fibrosis, and matrix remodeling in livers of Ppara−/−/gsta4−/− double knockout (dKO) mice fed chow, 35%, or 70% polyunsaturated fat (HF) diets. A: Representative immunohistochemistry of 4-hydroxynonenal (4-Hne) adducts in liver sections. B: Quantification of Hne protein adducts. C: Cyp2e1 mRNA expression. D: Nox2 mRNA expression. E: Representative sections of picrosirius red staining of collagen in HF livers (oil immersion objective). F: α Collagen (Col1a1) mRNA expression. G: Platelet-derived growth factor receptor b (Pdgfrb) mRNA expression. Statistical significance was determined by one-way analysis of variance followed by Student-Newman-Keuls post hoc analysis. Data are expressed as means ± SEM (B–D, F, and G). n = 5 mice (chow); n = 7 mice (35% or 75% HF). ∗P < 0.05 for chow versus HF diet; †P < 0.05 for 35% HF diet versus 70% HF diet. Original magnification, ×100 (E). Nox, NADPH oxidase.