Figure 6.

In vivo miR-24 inhibition decreases growth. A: Ten weeks after Mz-ChA-1 implantation into the flanks of nude mice, there was a significant reduction in tumor size in athymic mice treated with the miR-24 hairpin inhibitor compared to scramble mismatch-treated mice. Significance is shown versus mice treated with scramble mismatch. B: Real-time PCR performed on total RNA isolated from both tumor groups revealed decreased expression of proliferative and angiogenic factors. Significance is shown versus tumor samples from mismatch-treated mice. Data are expressed as means ± SEM of tumor size evaluations. n = 4 mice per each group (A); n = 3 experiments (B). ^∗P < 0.05 versus scramble mismatch control. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.