Figure 7.

Traumatic optic neuropathy (TON)-induced CXCL10 up-regulation is modulated by STAT signaling. A: Wild-type (WT) mice were subjected to TON, and proteins were isolated from retinas at indicated time points after TON. The phosphorylation levels of STAT1, STAT2, STAT3, STAT5, and STAT6 were evaluated by Western blotting. α-Tubulin was used as loading control. B and C: STAT1/STAT3 inhibitor Stattic or vehicle (Veh) was injected intravitreally into WT mice at 1 hour after the induction of TON. Retinas were collected at 4 hours after TON. B: Phosphorylated and total STAT1 and STAT3 were assessed by Western blotting. C: The expression of CXCL10 mRNA was analyzed by quantitative PCR. n = 5 mice (A–C). ^∗P < 0.05, ^∗∗P < 0.01.