Figure 8.

Myc-EH_ITSN expression leads to activation of p38^MAPK as well as downstream Elk-1 transcription factor and c-fos expression in K0^ITSN+/− mouse lungs. A: Lung lysates of untreated wt mice (white bars), K0^ITSN+/− mice (grey bars), and myc-EH_ITSN-transduced K0^ITSN+/− mice (black bars) were assessed for p38^MAPK phosphorylation. Total p38 kinase was used as a loading control. Densitometry values are representative for three independent experiments. B: Nuclear extracts prepared from controls and treated mice were assayed for Elk-1 activity. Data are representative of three independent experiments performed in triplicate. C: Western blotting of nuclear extracts (45 μg per lane) from controls and myc-EH_ITSN-transduced K0^ITSN+/− mice using c-Fos antibody and consequent densitometry analysis. Data were normalized to β-actin. Data represent three different experiments. Data are expressed as means ± SEM (A–C). n = 3 mice per group (A–C). ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗∗P < 0.005 versus wt mice.