Table 1.

Quantification of Pulmonary Arteries Remodeling: Percentage of Vessels Affected

Mouse model/remodeling	wt	wt + EHITSN	KDITSN or K0ITSN+/−	KDITSN or K0ITSN+/− + EHITSN
Obliteration (slit-like or no lumen)		8.0 ± 3.2		60.0 ± 8.0
Muscularized (irregular or complete layer of muscle)	5.3 ± 0.8	5.0 ± 0.7	6.0 ± 0.8	30.0 ± 4.5
Nonmuscularized	94.7 ± 2.1	80.0 ± 7.5	94.0 ± 9.5	10.0 ± 2.6

Data are shown as means ± SEM. If obliteration and muscularization affected the same pulmonary artery, only obliteration was counted. Quantification of affected vessels was performed on small- and medium-sized blood vessels (20 μm ≥ diameter ≤ 100 μm), as above, using three sections per mouse, three mice in the control group and five mice in the experimental group, with the experiments performed at least three times with reproducible results.

EH_ITSN, intersectin-1s fragment with endothelial cell proliferative potential; K0, knockout/heterozygous; KD, knockdown; wt, wild type.