Skip to main content
. 2017 Feb;187(2):377–389. doi: 10.1016/j.ajpath.2016.10.016

Table 1.

Oligonucleotide Sequences Used for Reverse Transcriptase PCR

Species Gene Forward sequence Reverse sequence
Human CAT 5′-CGTGCTGAATGAGGAACAGA-3′ 5′-TCTTCATCCAGTGATGAGCG-3′
qPCR human CAT 5′-ACGGGGCCCTACTGTAATAA-3′ 5′-AGATGCAGCACTGGAAGGAG-3′
Human GPX1 5′-CTTACAGTGCTTGTTCGGGG-3′ 5′-GTTCTTGGCGTTCTCCTGAT-3′
Human GADD45 5′-AAAGGATGGATAAGGTGGGG-3′ 5′-TCCCGGCAAAAACAAATAAG-3′
Human SOD2 5′-AATCAGGATCCACTGCAAGG-3′ 5′-AAGGCATCCCTACAAGTCCC-3′
qPCR human SOD2 5′-TAGGGCTGAGGTTTGTCCAG-3′ 5′-GGAGAAGTACCAGGAGGCGT-3′
Human CDKN1B 5′-TCACTCCAGGTAGTTTGGGG-3′ 5′-CACAAAACATGCCACTTTGG-3′
Human PRKG2 5′-GAGCGAACTGGAGGAAGAC-3′ 5′-GGTACACAACTTCACACCTTCT-3′
qPCR human PRDX3 5′-GACGCTCAAATGCTTGATGA-3′ 5′-GATTTCCCGAGACTACGGTG-3′
Human HPRT1 5′-GATGAAGAGCAAGGTTATGAC-3′ 5′-ACACAGAGCAACGATATGG-3′
qPCR human ACTB 5′-TGAAGGTGACAGCAGTCGGTTG-3′ 5′-GGCTTTTAGGATGGCAAGGGAC-3′
Mouse Cat 5′-GGACGCTCAGCTTTTCATTC-3′ 5′-AGAAGCCCGGATTATCGTTT-3′
Mouse Gpx1 5′-GTGGCGGGTTCGAGCCCAATT-3′ 5′-GCCAGGGCCGCCTTAGGAGT-3′
Mouse Gadd45 5′-TTCTGCTGCGAGAACGACAT-3′ 5′-AGTCATCTCTGAGCCCTCGT-3′
Mouse Sod2 5′-AAGGAGCAAGGTCGCTTACA-3′ 5′-CAAAGTAGTTGGCCTAAGAATAGATTT-3′
Mouse Cdkn1b 5′-AGATACGAGTGGCAGGAGGT-3′ 5′-TGTTTACGTCTGGCGTCGAA-3′
Mouse Prkg2 5′-GTGGCGGAGGACGCCAAGAC-3′ 5′-AGGCCTGCAGTGGGCTTCCT-3′
Mouse Prdx3 5′-TCGTCAAGCACCTGAGTGTC-3′ 5′-CACTCGGGGAGAATTGGTTA-3′
Mouse Gsr 5′-GCTCTGGGCCATTCGACGGG-3′ 5′-GAAGTCGGCCTTGGTGGCCC-3′
Mouse Actb 5′-CTGAGAGGGAAATCGTGCGT-3′ 5′-AGCTCAGTAACAGTCCGCCT-3′

qPCR, real-time quantitative PCR.