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. 2017 Mar;187(3):517–527. doi: 10.1016/j.ajpath.2016.11.004

Figure 2.

Figure 2

Reduced RPE65 levels in D477G KI mice. A: Relative levels of the RPE65 mRNA in the eyecups of these mice were analyzed by real-time PCR. Representative values are normalized to mRNA levels of a housekeeping gene (Gapdh). B: Eyecups containing sclera, choroid, and RPE were dissected and homogenized. The eyecup homogenates from wild-type (WT/WT), the heterozygous KI (WT/KI), and the homozygous KI (KI/KI) mice were then subjected to Western blot analysis with an antibody against RPE65 (PETLET). β-Actin was used as loading control. Densitometry was used for semiquantification. C: Immunofluorescence labeling of RPE65 on the retinal cross sections. RPE65 is shown in red, and nucleus is shown in blue (DAPI). Error bars represent SDs from the triplicates within the experiment (A). n = 3 per group (A); n = 4 per group (B). ∗∗∗P < 0.001 (t-test). Scale bar = 50 μm (C). GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; KI, knock in; ONL, outer nuclear layer; OPL, outer plexiform layer; OS, outer segment; RPE, retinal pigment epithelium; WT, wild type.