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. 2017 Mar;187(3):517–527. doi: 10.1016/j.ajpath.2016.11.004

Figure 3.

Figure 3

Analysis of opsin expression and localization. A: Retinal cross sections from 3.5-month-old WT/WT and WT/KI mice were immunolabeled with antibodies against short-wavelength cone opsin (S-opsin, yellow), midwavelength cone opsin (M-opsin, red), or rhodopsin (green). Nuclei were counterstained with DAPI (blue). B: Light microscopic images of the retinal cross sections from 13-month-old WT/WT and WT/KI mice. C: Transmission electron microscopy images of 13-month-old WT/WT and WT/KI. An unusual accumulation of oil droplet–like organelles in the RPE of WT/KI mice are indicated by arrowheads. Scale bars: 50 μm (A); 40 μm (B); 10 μm (C). GCL, ganglion cell layer; INL, inner nuclear layer; IS, inner segment; KI, knock in; ONL, outer nuclear layer; OPL, outer plexiform layer; OS, outer segment; RPE, retinal pigment epithelium; WT, wild type.