Figure 5.

Retinoid profile and isomerase activity in D477G KI mice. A: High-performance liquid chromatography (HPLC) retinoid profile analysis on the fully dark-adapted eyeballs from WT/WT and WT/KI mice. Both 11-cis-retinal (11-cis-RAL) and all-trans-retinyl ester (all-trans-RE) levels were measured. B: Isomerase assay using the eyecups from indicated mice. Levels of the generated 11-cis-retinol were measured by HPLC. The eyecup homogenates used for isomerase assay were examined by Western blot analysis to ensure the comparable amount of the total protein. Data are given as means ± SEM (A and B). n = 4 (A); n = 6 (B). ^∗P < 0.05 (t-test). KI, knock in; WT, wild type.