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. Author manuscript; available in PMC: 2018 Apr 7.
Published in final edited form as: J Mol Biol. 2017 Feb 27;429(7):999–1008. doi: 10.1016/j.jmb.2017.02.017

Figure 4.

Figure 4

Selected HDXMS uptake plots of regions of NFκB that exchange more upon DNA binding are plotted with free NFκB (black circles) vs. DNA-bound NFκB (green circles) or IκBα-bound NFκB (blue circles). The corresponding regions within NFκB are shown on a model of the DNA-bound NFκB (RelA-p50) structure. The model is colored according to a rainbow scale (blue-green-yellow-orange-red) representing the difference in fractional deuterium uptake between NFκB in complex with DNA as compared to NFκB alone. Boxes around each plot match to the color of the corresponding region in the structure. Red corresponds to 15% higher exchange in the NFκB-DNA complex and blue corresponding to 30% lower exchange in the DNA-bound form. The deuterium uptake plots are positioned near the corresponding structural regions and are also marked by arrows.