TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant characteristics | Source or reference |
|---|---|---|
| Serovar Typhimurium strains | ||
| SL1344 | hisG; virulent mouse strain; Smr | 12 |
| 12023 | Virulent mouse strain | 21 |
| GVB311 | SL1344 rpoE::Kmr | 13 |
| GVB1340 | 12023 ΔdegS::Kmr | This study |
| GVB1362 | SL1344 ΔdegS::Kmr | This study |
| Plasmids | ||
| pKD4 | pANT-Sγ derivative containing an FRT-flanked Kmr gene; Apr | 7 |
| pKD46 | pINT-ts derivative containing araC-ParaB and γβ exo DNA fragments; Apr | 7 |
| pTL61t | Low-copy-number, promotorless lacZ fusion vector; Apr | 18 |
| prpoEP3 | pTL61t with HindIII/XbaI-cloned RpoE-dependent rpoE promoter; Apr | 19 |