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. Author manuscript; available in PMC: 2017 Apr 12.
Published in final edited form as: Adv Exp Med Biol. 2012;758:149–155. doi: 10.1007/978-94-007-4584-1_21

Fig. 21.2.

Fig. 21.2

Morphometric assessment of NEB frequency, number and size in lungs of PHD-1deficient and WT control lungs using immunoperoxidase (a, b, c, d) and immunofluorescence (e, f, g, h) methods. (a) Comparison of mean values of %immunostained (SV2 antibody) area (IMS)per mm2 of section from PHD1- deficient and WT controls at different ages (P2 and 2 month). (b) Mean values for NEB size from same samples as in a). (c) Means of % IMS for NEB immunostained for CGRP as in samples shown in (a) & (b). (d) Means of NEB size in samples immunostained for CGRP as in samples shown in (a) & (b). (e) Comparison of mean number of synaptophysin immunoreactive NEBs/cm2 of airway in lungs of PHD-1 deficient mice and WT controls at P2 and P15. (f) Mean values for NEB size from same samples as in (e). (g) & (h) Same samples as in (e) & (f) with values expressed as IMS %of NEB (g) and NEB size /integrated area (h)