TABLE 1.

Primers used for construction of mutants

Primer	DNA sequence (5′ → 3′)a	Application	Amplicon (bp)
ropA55′	ACTATGTCGTGACACCAGCATC	5′ fragment for TW90	415
ropA53′	TAGAGCTCTTCTTTCACCAGATATGCTC	5′ fragment for TW90
ropA35′	ATAAGCTTATCGTAAATGTAACAACACCAC	3′ fragment for TW90	512
ropA33′	AGCAAACATGGAACGCAGAGC	3′ fragment for TW90
TF-P1	AAAGAAGTTTTGCCAATCGC	5′ fragment for SMUTig	781
TF-P2	GGCGCGCCTCGTAAATGTAACAACACCACG	5′ fragment for SMUTig
TF-P3	GGCCGGCCCATGGAAGCTGAGCAAGTGCG	3′ fragment for SMUTig	718
TF-P4	ATTTATCAAGGCATGACGGGG	3′ fragment for SMUTig
TF5′	ATGGATCCGGCTGCAACTACACCGACTGC	Internal fragment for TW90A	595
TF3′	GAGTCGACCCGATTCTACCGAAGAGCTCGGTATC	Internal fragment for TW90A

^aThe incorporated restriction sites are underlined.