Figure 8.
Analysis of mtDNA copy number, steady-state levels of mature mt-RNAs and mitochondrial protein synthesis in WT and Δmpa1 cells. (A) Determination of mtDNA copy numbers in WT and Δmpa1 cells by qPCR. (B) qRT-PCR analysis of the steady-state levels of mt-RNAs in WT and Δmpa1 cells. (C) Northern blot analysis of mature mt-RNAs from WT and Δmpa1 cells. (D) In vivo labeling of mitochondrial translation products with [35S]-methionine/cysteine. (E) Deletion of mpa1 affects steady-state mtDNA-encoded protein levels. The experiments were performed as in Figures 2 and 3.