Table 1. pol β NLS dissociation constants.
| Ligand | Target | Kd (μM) |
|---|---|---|
| SKRKAPQETLNGG[Lys(FITC)]a | mImpα1ΔIBB | 3.6 ± 0.4 |
| SKRKAPQETLNGG[Lys(FITC)]a | mImpα1 | 61.8 ± 1.7 |
| SKRKAPQETLNGG[Lys(FITC)]a | mImpα1ΔIBB (W357R,W399R) Minor site blocked | 225 ± 11 |
| SKRKAPQETLNGG[Lys(FITC)]a | mImpα1ΔIBB (W184R,W231R) Major site blocked | 4.5 ± 0.5 |
| SKRKAPQETLNGG[Lys(FITC)]a | Impα5ΔIBB | 0.720 ± 0.030 |
| Pol β lyase domainb | mImpα1ΔIBB | 0.140 ± 0.017 |
| Pol β(R4S,K5S) lyase domainb | Impα5ΔIBB | 530 ± 2 |
| Pol β lyase domainb | Impα5ΔIBB | 0.017 ± 0.006 |
aKd values determined by fluorescence polarization.
bKd determined by ligand displacement.