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. 2016 Dec 9;45(5):2531–2545. doi: 10.1093/nar/gkw1241

Figure 1.

Figure 1.

And-1 forms complexes with HR repair proteins and is required for HR repair. (A) Identification of CtIP-associated proteins that are involved in HR repair by mass spectrometry. (B) CtIP interacts with And-1. Co-immunoprecipitation (co-IP) assays were performed using U2OS cell lines. Cell lysates were immunoprecipitated with control IgG, anti-CtIP or anti-And-1 antibodies and the IPs were then resolved by SDS-PAGE and immunoblotted for the indicated proteins. Left panel, immunoprecipitation with anti-CtIP antibody. Right panel, immunoprecipitation with anti-And-1 antibody. (C) And-1 depletion impairs HR repair. U2OS-DR-GFP cells treated with the indicated siRNAs were transfected with pCBASce plasmids 48 h post siRNA transfection. The percentage of GFP-positive cells was determined by flow cytometry 48 h after plasmid transfection. The data were normalized to those obtained from cells transfected with control siGl2 (set as 1.0). Data represent means ± SD from three independent experiments. *P ≤ 0.05. (D) U2OS cell survival after exposing cells transfected with indicated siRNAs to the indicated doses of camptothecin. Data represent means ± SD from three independent experiments. *P ≤ 0.05; **P ≤ 0.01.