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. 2016 Dec 9;45(5):2531–2545. doi: 10.1093/nar/gkw1241

Figure 5.

Figure 5.

And-1 depletion impairs DNA damage response induced by end resection. (A and B) Depletion of And-1 impairs Chk1 and RPA phophorylation but not Chk2 phosphorylation after camptothecin exposure. U2OS cells transfected with the indicated siRNAs were treated with camptothecin (1 μM) for 1 h. Cells were then harvested and immunoblotted for indicated proteins. Asterisks in A and B: hyper-phosphorylated RPA32. (C) Depletion of And-1 impairs Chk1 phosphorylation at both early and late time-points after continuous camptothecin exposure. U2OS cells transfected with the indicated siRNAs were treated with camptothecin (1 μM) and harvested at the indicated time-points and immunoblotted for the indicated proteins. (D) U2OS cells transfected with indicated siRNAs were synchronized by using a double-thymidine (2 mM) block and then released for 6h. Cells enriched in S/G2 phase were harvested for IP. CtIP or IgG IPs were immunoblotted for the indicated proteins.