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. 2016 Dec 9;45(5):2531–2545. doi: 10.1093/nar/gkw1241

Figure 7.

Figure 7.

Recruitment of And-1 to DSB sites is dependent on BRCA1, MDC1 and ATM but not on MRN complex. (A) Depletion of BRCA1, or MDC1 but not NBS1 impairs the localization of And-1 to DSB sties. U2OS cells transfected with the indicated siRNAs were treated as in Figure 2A. The percentage of γ-H2AX cells exhibiting And-1 strips was determined. Data represent means ± SD from three independent experiments. N.S., not significant; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. (B) Localization of And-1 to DSB sites is restored in HCC1937 cells expressing wild type BRCA1. HCC1937 or HCC1937 cells expressing wild-type BRCA1 were treated as in Figure 2A. The percentage of γ-H2AX cells exhibiting And-1 strips was determined. Data represent means ± SD from three independent experiments. **P ≤ 0.01. (C) Inhibition of ATM impairs the localization of And-1 at DSB sites. U2OS cells treated with KU-55933 (10 μM) for 4 h were treated as in Figure 2A. The percentage of TopBP1 cells exhibiting And-1 strips was determined. Data represent means ± SD from three independent experiments. **P ≤ 0.01.