FIG. 2.

Protein dot blot with recombinant Fbp from E. canis incubated under native conditions (N), under denaturing conditions with 0.1% SDS, under reducing conditions with 50 mM dithiothreitol (DTT), or in the presence of both SDS and DTT. Serum samples from E. canis-infected dogs (rows 1 to 3) were collected on day 28 (A) and day 42 (B) postinoculation. A recombinant protein expression control (C) with chloramphenicol acetyltransferase expressed by the same expression vector was used as a negative control.