Figure 2.

HMGNs regulate the expression of OLIG1 and OLIG2 in ESCs. (A) Regulatory network of genes down-regulated during DKO ESC differentiation and attributed to ‘Neuron Differentiation’ GO category. (B and C) RNA-seq analysis of Olig1&2 expression during ESCs differentiation. Data were obtained from three independent ESC clones (biological replicates) and presented as mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001). (D) Westerns showing OLIG1&2 protein expression in WT and DKO ESCs. (E) Westerns showing siRNA mediated depletion of HMGNs down regulated OLIG&2 expression. (F) Rescue of OLIG1&2 expression by re-introducing either wild type (green) or mutant (red) HMGN in DKO ESCs. Shown are representative blots from at least two independent experiments.