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. 2016 Dec 6;45(6):3031–3045. doi: 10.1093/nar/gkw1222

Figure 5.

Figure 5.

Loss of HMGNs affects oligodendrocyte differentiation in vitro. (A) Schematic diagram of the protocol for in vitro differentiation of ESCs toward embryoid body (EB), neural progenitor (NP), and oligodendrocyte precursor cells (OPCs). (B) Representative immunofluorescence images showing equal Nestin expression in WT and DKO NPs. (C) Quantification of Nestin positive cells, over 250 cells from 10 random regions were counted. (D) Immunofluorescence showing lower frequency of OLIG2 positive cells in DKO OPCs. (E) Quantification of OLIG2 positive cells, over 250 cells from 10 random regions were counted. All the data are presented as mean ± SEM (****P < 0.0001).