FIG. 3.

DC activated with 0-3hRP polarize naïve CD4⁺ cells toward a Th2 phenotype in vitro. Differentially activated mDC (stimuli as given in the legend to Fig. 1) were cocultured with CD4⁺ cells from DO11.10 mice in the presence of OVA peptide (10 nM). Culture supernatants were analyzed by ELISA for IFN-γ and IL-4. Data are the mean ± standard error of the mean of the log increase (n-fold) in cytokine production primed by test groups relative to DC-medium and are derived from three experiments. The peaks of cytokine production varied between experiments but were detected in the following ranges: IFN-γ, 2,500 to 8,000 pg/ml, and IL-4, 800 to 1,600 pg/ml. Levels of significance are between DC-medium and the DC test groups.