FIG. 4.

DC activated with 0-3hRP drive polarization toward a Th2 phenotype in vivo. Naïve DO11.10 mice were inoculated subcutaneously with differentially activated, OVA-pulsed DC (stimuli as given in the legend to Fig. 1). After 7 days, sdLN cells were restimulated in vitro with OVA (0 to 10,000 nM) for 72 h. Culture supernatants were analyzed by ELISA for IFN-γ, IL-4, IL-5, and IL-10. Data are the mean plus standard error of the mean of three individual wells and are representative of four experiments. Levels of significance are between DC-medium and the DC test groups. N.D., not done.