FIG. 5.

DC activated with 0-3hRP drive systemic Th2 polarization. DO11.10 mice were inoculated subcutaneously with differentially activated, OVA-pulsed DC (stimuli as given in the legend to Fig. 1). After 7 days, splenocytes were restimulated in vitro with OVA (0 to 1,000 nM) for 72 h. Supernatants were analyzed by ELISA for IFN-γ, IL-4, IL-5, and IL-10. Data are the mean plus standard error of the mean of three individual wells and are representative of three experiments. Levels of significance are between DC-medium and the DC test groups.