FIG. 6.

Ligation of CD40 increases IL-12 production by DC stimulated with 0-3hRP. DC-medium or DC plus 0-3hRP were matured in the presence or absence of αCD40 MAb (5 μg/ml) (a) or on a monolayer of CD154⁺ or control fibroblasts (b). Culture supernatants were tested by ELISA for IL-12p40, IL-12p70, IL-6, and IL-10; are shown as the mean plus standard error of the mean of three or more wells; and are representative of four experiments. Levels of significance are between untreated DC-medium or DC plus 0-3hRP and the respective DCs cultured with CD154⁺ fibroblasts or αCD40 MAb. The dotted line represents the lower limit of ELISA detection.