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. 2016 Nov 28;45(4):1731–1742. doi: 10.1093/nar/gkw1145

Figure 2.

Figure 2.

L4-22K binds preferentially to the sense strand R1 ssDNA and ssRNA. (A) The 5΄-end labeled double stranded R1 ssDNA probe (0.4 pmol) was incubated with 0.1 μg of recombinant L4-22K protein (lanes 2–10), together with a 2.5, 5, 7.5 or 10-fold excess of an unlabeled single strand sense (lanes 3–6) or antisense (lanes 7–10) R1 ssDNA competitor. (B) The radiolabeled R1 dsRNA probe (0.4 pmol) was incubated with 0.09 μg of recombinant L4-22K protein (lanes 2–10), together with a 2.5-, 5-, 7.5- or 10-fold excess of the unlabeled sense (lanes 3–6) or antisense (lanes 7–10) R1 ssRNA competitor. ‘–’ denotes no recombinant L4-22K protein added. The experiments shown in panels A and B have been conducted three times.

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