Figure 8.

L4-22K overexpression suppresses HAdV-5 capsid protein synthesis and accumulation in vivo. HEK293 cells were transfected with increasing concentration of pcDNA3-Flag-L4-22K, while total amount of DNA was kept constant in all transfected sample with an empty plasmid. Twenty four hours later, cells were HAdV-5- (10 FFU/cell) or mock-infected for another 18 h followed by ³⁵S-methionine/cysteine pulse labeling for 2 h and total proteins were extracted. (A) Rate of protein synthesis was visualized by autoradiography, accumulation of hexon late viral protein is demonstrated by arrow. (B) Immunoblotting from the same samples against capsid proteins, Flag-L4-22K and Actin.