Figure 5.

Characterization of nucleosomal arrays with different nucleosome occupancies. (A) Characterization of saturated 601_207×13 nucleosomal arrays (13) and arrays missing a single nucleosome in random positions (13-1RN), a central nucleosome (13-1CN), or up to two nucleosomes (13-1RN-1CN). The templates were characterized using the restriction enzyme sensitivity assay (Figure 1B). Analysis of end-labeled DNA by denaturing PAGE (right). (B) The nucleosome-free region of the 13-1CN nucleosomal array is not protected by a nucleosome (left). Chromatin was digested with an excess of restriction enzyme CacAI. DNA was purified, digested by enzyme EcoRI, and subjected to primer extension. Analysis of end-labeled DNA by denaturing PAGE (right).