Figure 7.
The presence of multiple nucleosome-free gaps decreases the rate of EPC. (A) Transcription of the (13-1R) and (13-1R-1C) nucleosomal arrays. Analysis of labeled transcripts by denaturing PAGE. Whereas the omission of one nucleosome in the 601207×13 array increases the efficiency of EPC, two missing nucleosomes decrease EPC. The rates of EPC were measured as described in Figure 3A. (B) Quantitative analysis of the specific transcripts shown in Figure 7A. Error bars indicate standard deviations based on four independent measurements using two different reconstitutes (see Supplementary Table S2 for the results of t-test).