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. 2017 Feb 8;45(6):3253–3265. doi: 10.1093/nar/gkx087

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Chromosomal de-condensation estimated by DNA FISH. Biotin- (orange) or DIG- (green) labelled probes targeted against the two flanking regions of either cluster #3 or #15 were used. (A) A tapetum cell with a pair of green and orange signals, marked by a square (cluster #15, DEX). (B) The inset (i) from micrograph (A) and distance measurement along the x-, y- and z-axis. (C–F) Micrographs showing examples of signals collected from: tapetum cells of MOCK- (C, E) and DEX- (D, F) treated samples, using the probes targeted against cluster #3 (C, D) or cluster #15 (E, F). (G) Violin plots showing the frequency distribution of the distance between Biotin- and DIG-labelled probes directed against the flanking regions of cluster #3 (left) and cluster #15 (right). Mock: negative control; DEX: dexamethasone treated samples. Size bars: 500 nm (B) and 100 nm (C to G).