Figure 7.

CNBP depletion impairs IL-6 production, causing defects of bacterial defense. (A–D) CNBP expression affects the LPS (80 ng ml⁻¹)- or CpG-DNA (1 μM)-induced production of IL-6, but not TNF-α. ELISA of IL-6 and TNF-α expression in culture supernatants of CNBP-depleted or -overexpressed RAW macrophages, BMDCs or BMDMs. *P < 0.05 and **P < 0.01 (Student's t-test). (E) CNBP depletion impairs mRNA expression of sustained inflammatory cytokines after bacterial infection. MEFs were transduced with retrovirus expressing either GFP shRNA or CNBP shRNA, and then cells were infected with Shigella flexneri for 1 h. The mRNA expression level of indicated genes was performed by RT-PCR. Band intensity from RT-PCR results was quantified using densitometry, and results were normalized to gapdh. *P < 0.01 (Student's t-test). Immunoblot or RT-PCR analysis of transduction efficiency (right panels of A–E). Data are representative of at least two independent experiments and are presented as mean ± s.d. in A–E.