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. 2016 Dec 8;45(5):2408–2422. doi: 10.1093/nar/gkw1186

Figure 5.

Figure 5.

SREBP1c and SP1 bind to the rs174557 locus in vivo to regulate FADS expression. (A and B) The expression of FADS1 and FADS2 in HepG2 cells were significantly increased by overexpression of SREBP1c detected by both RT-qPCR (A) and western blot (B). The qPCR results are expressed as mean±SD from three replicates with **P < 0.01 and ***P < 0.001 calculated by two-tailed t tests. (C) The allelic expression imbalance of FADS1 in MCF7 cells was significantly decreased by SREBP1c overexpression. The results came from four replicates with the error bars represent SD. (D–G) The rs174557 locus is bound by both SREBBP1 and SP1 in vivo detected by ChIP-qPCR with antibody against SREBP1 (D) and SP1 (E) in HepG2 cells. The binding of SREBP1 (F), but not SP1 (G) to the rs174557 locus was significantly increased by SREBP1c overexpression. The region F2-pro-SRE targeting the SREBP1 responsive region in the FADS2 promoter was used as the positive control for both SREBP1 and SP1 binding. All the results are expressed as fold enrichments over the negative control region GDCHR12 with three to six replicates. Error bars represent SD. ns, not significant; *P < 0.05; **P < 0.01 and ***P < 0.001 compared with GDCHR12.