Skip to main content
. 2016 Dec 8;45(5):2408–2422. doi: 10.1093/nar/gkw1186

Figure 7.

Figure 7.

Competition between PATZ1 and SP1 for the overlapping GC-box element determines the extent of the rs174557 locus to be induced by SREBP1c. (A) The alleles of rs174557 respond differently to SREBP1 induction in luciferase assay. Abbreviations are as follows: Chimp, the chimpanzee allele; and con, the consensus sequence of AluYe5 element. ***P < 0.0001 compared with allele D of rs174557. (B) PATZ1 determines the degree of SREBP1c induction on allele A2 of rs174557 in a dosage dependent manner. A gradual increase of PATZ1 expression leads to a gradual decrease in enhancer activity. Disrupting the PATZ1 binding site (A2-PT1 mut) completely abolished the suppression by PATZ1. ***P < 0.0001 by one-way ANOVA when compared with allele A2 of rs174557 with only SREBP1c overexpressed. All the data in A and B came from three to four independent transfections each with six technical replicates. The bars represent mean±SD from all technical replicates. (C) Model of how FADS1 is regulated by PATZ1 binding to rs174557 with different affinities between alleles.