Table 1. Mass spectrometry analysis of cellular ribonucleases that co-purify with Cas10–Csm(Csm2H6N) in S. epidermidis and their putative functions.
Ribonuclease | Theoretical mass (kDa) | Unique peptide count | Normalized spectral countse |
---|---|---|---|
PNPasea, b | 77 | 28 | 74.55 |
Ribonuclease J1c | 62 | 10 | 21.3 |
Cbf1a, b | 36 | 7 | 19.53 |
Ribonuclease J2c | 63 | 10 | 16.86 |
RNase IIId | 28 | 5 | 12.43 |
RNase Ra | 91 | 5 | 7.1 |
a3΄-5΄ exoribonuclease on single-stranded RNA.
b3΄-5΄ exonuclease on single-stranded DNA.
c5΄-3΄ exonuclease on single-stranded RNA.
dendoribonuclease on double-stranded RNA.
eValues reflect relative protein abundance.