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. 2016 Sep 28;45(4):2051–2067. doi: 10.1093/nar/gkw860

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Association of single U2 snRNPs on globin-derived pre-mRNA with two introns (CEC) under conditions favoring accumulation of complex A. (A) Frequencies of pre-mRNA complexes in which fluorescent factors bleached in the indicated number of steps after incubation of the RNA in nuclear extract either depleted of ATP (−ATP) or in the presence of ATP and oligonucleotide complementary to U6 snRNA (+ATP). (B) Frequencies of bleaching of mEGFP-U2B″ in one or two steps for CEC complexes formed after incubation for the times indicated in the presence of ATP without oligonucleotide complementary to U6 snRNA. (C) Time course of splicing of CEC pre-mRNA. The mRNA products are indicated.