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U2 snRNP association in the absence of ATP is the same as in the presence of ATP when phosphatases are inhibited. Histograms show the numbers of steps in which U2AF35, U2AF65 and U2 snRNP associated with pre-mRNA bleach after incubation in nuclear extracts depleted of ATP but containing phosphatase inhibitors. (A) GloC pre-mRNA. (B) GloC-2×3΄ss pre-mRNA. (C) CEC pre-mRNA.
