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. 2016 Sep 28;45(4):2051–2067. doi: 10.1093/nar/gkw860

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Effects of repressors and activators on recruitment of 3΄ss factors. (A) Effects of mutations (crosses) in PTB-binding sites on association of U2AF35 and U2AF65 with pre-mRNA containing the PTB-regulated exon 3 of tropomyosin (Tpm1). The construct TM1 contained the 5΄ exon and adjacent 65 nts of the intron of Glo (64), fused to 314 nts of Tpm1, comprising 188 nts of intron 2 and the whole of exon 3. In TM4, the three UCUU motifs had been mutated to UUUU (62). The extract had been pre-incubated to remove ATP. (B) Association of 3΄ss factors with pre-mRNA containing exon 7 of SMN1 or SMN2. Frequencies of bleaching steps are shown for each pre-mRNA incubated either in nuclear extract pre-incubated to deplete ATP (upper part) or in nuclear extract in conditions favoring the accumulation of complex A.