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. 2016 Dec 12;45(5):2558–2570. doi: 10.1093/nar/gkw1249

Figure 1.

Figure 1.

Dia2 is essential for restart of stalled replication forks. (A) Ten fold serial dilutions of the indicated strains were spotted on yeast extract-peptone-dextrose (YPD), YPD + 0.007% methyl methane sulfonate (MMS) or 200 mM hydroxyurea (HU) and incubated at 30°C. (B) Equal number of cells were plated on media containing the indicated amounts of MMS or HU and colony-forming units were counted after 4 days at 30°C. Error bars represent standard deviations from three independent experiments. (C) Schematic of DNA fiber assay depicting sites of replication. Red tracts, IdU; green tracts, CldU. (D) Representative figure showing inability to restart replication after 0.05% MMS treatment in absence of Dia2, Sgs1 or Mph1. (E) Dia2, Sgs1 and Mph1 work in concert to mediate replication fork restart after MMS-induced fork stalling. The replication restart efficiencies of wild type, dia2Δ, sgs1Δ, mph1Δ, dia2Δsgs1Δ and dia2Δmph1Δ strains were measured as the number of restarted replication forks (IdU–CldU tracts) compared with the total number of IdU-labeled tracts (IdU only tracts plus IdU–CldU tracts). ***P < 0.001.