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Mph1 protects nascent DNA strands from nucleolytic degradation after fork stalling. Nascent replication fork tract lengths (labeled with IdU only) were measured after 1 h of 0.05% MMS treatment in dia2Δ, sgs1Δ, mph1Δ, dia2Δsgs1Δ and dia2Δmph1Δ strains. P < 0.05 for wild type versus dia2Δ, sgs1Δ or dia2Δsgs1Δ and P < 0.001 for wild type versus mph1Δ or dia2Δmph1Δ strains.
