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Upon stalled fork recovery, Dia2 dissociates from Sgs1. Wild-type cells were either untreated or treated with 0.05% MMS for 20 or 40 min and allowed to recover in the fresh media for 20, 40 and 60 min. Whole cell lysates were prepared and used in immunoprecipitations with an anti-myc antibody (lanes 1–9) or protein A/G beads without antibody (lanes 10–18). Immunoblots were probed with anti-myc or anti-Sgs1 antibodies.
