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. 2016 Dec 12;45(5):2558–2570. doi: 10.1093/nar/gkw1249

Figure 7.

Figure 7.

Depletion of Mrc1 in dia2Δ strains rescues deficiencies in fork restart and maintenance of replication velocity by recruiting Sgs1 to chromatin. (A) Replication fork restart efficiencies of wild-type and a dia2Δmrc1 degron strain with or without IAA were measured as number of restarted forks (IdU-CldU tracts) compared with the total number of IdU-labeled tracts (IdU only plus IdU–CldU tracts). ***P < 0.001, NS = not significant. (B) CldU tract length distributions were determined in wild type, and a dia2Δmrc1 degron strain with or without IAA. P < 0.05 for wild type versus dia2Δmrc1 degron - IAA, and P-value is not significant for wild type versus dia2Δmrc1 degron + IAA. (C (i) and (ii)) Crude pellet containing chromatin fractions were separated from soluble cytoplasmic fractions from the untreated (lanes 1–6) or DNaseI-treated (lanes 7–12) whole cell lysates of wild type and a dia2Δmrc1 degron strain with or without IAA. Samples were resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotted for Dia2Myc, Mrc1HA and Sgs1 using anti-Myc, anti-HA or anti-Sgs1 antibodies, respectively. S, soluble cytoplasmic fraction; P, pellet containing chromatin fraction.